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KMID : 0614020080230010070
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Journal of Pharmaceutical Sciences (C.N.U.)
2008 Volume.23 No. 1 p.70 ~ p.75
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Change of Human Salivary α-Amylase Activity and Free Radical Scavenging Activity after Psychological Stress
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Hung Nguyen-Dang
Sok Dai-Eun
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Abstract
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Salivary a-amylase activity(sAA) and total salivary free radical scavenging activity(FRSA) has been Suggested as effective stress markers. In the present study, we measured sAA and FRSA, which were induced by test stress. Saliva samples were collected from 15 healthy volunteers, and their sAA and FRSA were determined by using blue dextran method and 1,1-diphenyl-2-picryIhyrazyl(DPPH) assay, respectively. Overall, there is no Significant difference of sAA between pre-stress and post-stress. Meanwhile, the average value of FRAS is Significantly decreased after test stress. Taken together, the ratio of sAA/FRSA is more significantly increased after stress, suggesting that the ratio of sAA/FRSA is more a reliable marker for the assessment of psychological stress.
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KEYWORD
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stress, saliva, amylase, FRSA
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